Geneious Prime provides several operations for designing and working with PCR Primers and DNA or hybridisation probes. PCR Primers and DNA or hybridisation probes can be designed for or tested on existing nucleotide sequences or alignments. A PCR product can be extracted from a sequence that has been annotated with both a forward and a reverse primer. 5’ extensions consisting of restriction enzymes or arbitrary sequence may also be added to primer documents.
In addition Geneious Prime can determine the primer characteristics for a primer sized sequence and convert it into a primer. Characteristics can also be determined for any number of primer sized selections made in the Sequence View.
To use any one of these primer operations simply select the appropriate nucleotide sequences and either select Primers from the Tools menu or right-click (Ctrl+click on Mac OS X) on the document(s) and select Primers. A popup menu will appear showing the operations valid for your current selection.