13.8 Convert to Oligo

Geneious Prime can convert nucleotide sequences into primers. This is necessary for sequences to show up in the oligo database.If your sequences are less than 36 bp long, this operation will also determine the primer characteristics of the sequences, such as melting point. To do this, select your sequences and choose Primers Convert to Oligo from the popup menu that appears. If you select just two sequences you have the additional option of determining their pair characteristics. Determining the pair characteristics of two primer sequences can be used to see if two sequences can pair and how well they do so.

Note: Sequences longer than 36 bp can still be converted into oligo sequences, but their characteristics cannot be calculated as this is the size limit that Primer3 sets for accurate Tm calculations.