The Characteristics section allows you to set absolute limits on properties of primers and probes such as melting point and GC content. Optimum values can also be speciﬁed. For details on individual options hover your mouse over them and a popup box will describe the function of the option.
Characteristics can be set for either Primers or DNA Probes, depending on the task you have chosen. The Primer section is available if one of Forward Primer or Reverse Primer is being designed or tested and DNA Probe is available if a DNA Probe is being designed or tested. These two sections are quite similar; the DNA probe section has a subset of the options available in the primer section. This is because primers are usually chosen in pairs and so several options can be set for how pairs are chosen.