13.1.1 Generic primers

This option will design standard PCR primers according to the region input options you select. These options allow you to specify what part of a sequence you wish to amplify. Most options are optional and can be enabled or disabled with the associated check boxes beside them. If you have selected a region in the sequence before opening the primer dialog then this region will automatically be used for Included Region and Target Region. All of these are expressed in base pairs from the beginning of the sequence and are as follows:

The final option in this section is Number of Pairs to Generate which specifies how many candidate pairs of primers and DNA probes to generate and is compulsory. Setting this to 1 will give you only the primer pair which was considered best by the set parameters.