If no primers or DNA probes that match the speciﬁed criteria can be found in one or more of the sequences then a dialog is shown describing how many had no matches and for what reasons.
To see why no primers or DNA probes were found for particular sequences, click the ‘Details’ button at the bottom of the dialog. The dialog will then open out to display a list of all the sequences for which no primers or DNA probes were found. For each of the sequences the following information is listed:
After examining the details you can choose take no action or continue and annotate the primer and/or DNA probes on the sequences which were successfully designed for.