When no primers can be found

13.1.10 When no primers can be found

If no primers or DNA probes that match the speciﬁed criteria can be found in one or more of the sequences then a dialog is shown describing how many had no matches and for what reasons.

To see why no primers or DNA probes were found for particular sequences, click the ‘Details’ button at the bottom of the dialog. The dialog will then open out to display a list of all the sequences for which no primers or DNA probes were found. For each of the sequences the following information is listed:

Which of Forward Primer, Reverse Primer, Primer Pair and/or DNA Probe could not be found in the sequence
For each of these, speciﬁc reasons for rejection are listed (eg. “Tm too high” or “Unacceptable product size”) along with a percentage which expresses how many of the candidate primers or probes were rejected for this reason.

After examining the details you can choose take no action or continue and annotate the primer and/or DNA probes on the sequences which were successfully designed for.

< Prev Up