To analyze the results of your CRISPR editing experiment, go to Annotate and Predict → Analyze CRISPR Editing Results. This operation measures the frequency of variants around the CRISPR editing site by mapping reads to the target sequence. Mapped reads are collapsed into clusters based on their difference from the reference and the frequency of each cluster is reported in the alignment (for a description of the algorithm, see 15.2.3 ).
This operation is designed for amplicon sequences produced from either Sanger or NGS sequencing. Sequences must be in a sequence list, and paired reads should be merged via menu Sequence → Merge Paired Reads prior to running this operation.