If there is a speciﬁc set of enzymes that you want to use repeatedly, you can create a custom enzyme set. There are two ways to do this:
New enzyme sets are created as documents with the icon. These are created in whatever folder you are working in, but all enzyme sets in your database are available in the Candidate Enzymes drop down list, in the Find Restriction Sites interface.
To create an enzyme set containing restriction enzymes which do not cut a particular sequence, select your sequence and go to Cloning → Find non-cutting enzymes. Select the criteria and the candidate enzymes you wish to test, and click OK. A new enzyme set document will then be created containing the enzymes which do not cut your sequence.