13.2.1 Manually pairing primers

If you have added your primers to a sequence using the manual selection method described above, or you have run Add Primers to Sequence (section 13.6 ) or Test with Saved Primers (section 13.5 ) without checking ”pairs only”, then your primers will be annotated on the sequence as individual primers, rather than associated with their pair. To pair individual primers after they are annotated, select both primers using cntrl/command-click, then right-click on one of primers and select Manually pair primers.


PIC


Figure 13.7: Manually pairing primers


You should then see a line linking the F and R primer, and pair characteristics (Pair dimer Tm and product size) will be added to both primer annotations.