Figure 13.10:
The “Add Primers to Sequnce” dialog
If you have primer sequences you’d like to test against a sequence but you don’t have the primer documents in your database already, you can use Add Primers to Sequence. This lets you enter a Name and Sequence for one or more primers, and gives you some basic options for testing. You can test multiple primers by adding more rows via the + buttons on the side.
Up to ten previously entered primer names and sequences are remembered in the corresponding fields to make it easier using the same primer again.
All specified primers that bind the selected sequence will be annotated on the sequence, as well as mismatch annotations, as appropriate. Geneious will automatically check the entire template for other binding sites, if any are found they will be annotated on the primer under Off-target sites. If Extract primers to folder is selected, all annotated primers will also be saved as separate primer documents to the folder containing the selected sequence.