Now that you have set up the executables, it is time to add databases to your BLAST.
To create a database from the sequences in a FASTA ﬁle, go to Tools → Add/Remove Data-bases → Add BLAST Database and select Custom BLAST from the Service drop-down box (Figure 15.5 ). Choose to Create from ﬁle on disk and then click Browse to navigate to the FASTA ﬁle that contains the sequences you want to BLAST. Enter a name for the database and click ‘OK’. There are two requirements for a FASTA ﬁle to be suitable for creating a database from:
If the ﬁle meets these requirements it will be added as a database, otherwise you will be informed of the problem.
To create a BLAST database from sequences in your local documents folders, ﬁrst select the documents that you want. Then go to Tools → Add/Remove Databases → Add BLAST Database and select Custom BLAST from the Service drop-down box. Enter a name for the database, and click ‘OK’.