Creation of a single primer sequence
If you want to create a primer, for example using a published primer sequence, go menu File → New → Sequence. This will open the New Sequence window, just enter the primer sequence and set Type to Primer. If the primer has an extension, select the region corresponding to the binding region, then hit the Binding region: button to set the binding region start point.
Hit OK and the new primer file will be created.
Alternately, you can add the extension after the primer is created. To do this, create a primer as above but do not include the extension. Once the primer is created, select the new primer and go Primers → Add 5' Extension. This will allow you to add annotated extension elements, such as restriction sites or Gateway sites, to your primer.
Use the link below to jump to Exercise 3.
Exercise 1: A Primer primer
Exercise 2: Manual entry of primers
Exercise 3: Importing tables of primers
Exercise 4: Manual design of primers
Exercise 5: Design of PCR primer pairs
Exercise 6: Testing primers against a target
Exercise 7: Degenerate primer design
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