In this tutorial you will learn how to perform a reference assembly with next-generation sequencing (NGS) data, and to call SNPs on the assembled contig. You will learn the typical steps of an NGS workflow such as quality trimming, read pairing, and generating a consensus sequence.
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Overview: NGS preprocessing best practice An overview of NGS preprocessing steps available in Geneious.
Exercise 1: NGS read Preprocessing In this exercise we show you how to pair and trim NGS reads.
Exercise 2: Map to Reference In this exercise we show you how to pair and trim NGS reads.
Exercise 3: Exploring contig documents
Exercise 4: Calling SNPs
Exercise 5: Comparing SNPs