Many experiments might require a more complex setup than only assembling two fragments to each other or inserting one sequence into a vector. Thus Gibson Assembly in Geneious handles batch operations with grouped sequence documents. Each sequence in such a group will be inserted into the same position, and a new product & corresponding primers will be generated for each alternating sequence.
In this example we want to test the expression of the DCN fusion gene with a variety of different promoters.
Select the DCN CDS and digested vector from the first exercise, plus the sequence list 'Promoter'. This is a list of 5 promoters downloaded from NCBI.
Start the Gibson Assembly operation (Cloning → Gibson Assembly...)
This time we have two sequences in the drag'n'drop field with the Promoter sequences in a brownish color. This indicates that it is a sequence list instead of a single sequence. We want the Promoters to be 5' of the DCN CDS, so drag it to the first position if it is not already there. Because we're expecting 5 products we want them to be saved in a subfolder this time. The other settings should still be remembered from the previous run.
Click OK and open the Report Document in the subfolder after the operation has finished. This time we see all the five products listed after the 'Hints' section, with only the promoter (Insert 1) changing. We have more primers, and when scrolling down we find 4 primers that are greyed out. This is because these are the primers between the DCN CDS and the vector, and they are the same for all 5 products, so only one of them has to be ordered. When scrolling through the primers you might notice that some of them are pretty short - the shortest primer binding site is only 11 bases long. So pay attention to this as you may have to manually adjust these primers or consider using different promoter sequences.