CRISPR tutorial

This tutorial was developed by Dr Helen Shearman (Biomatters Ltd, New Zealand)

In this tutorial you will use the CRISPR tool in Geneious to search for "GN(20)GG" guide RNA (gRNA or "CRISPR") sites in the LYP1 gene from Saccharomyces cerevisiae (baker's yeast) and check for non-target binding sites against the rest of the Saccharomyces cerevisiae geneome.


Introduction Introduction
Exercise 1: Finding CRISPR sites
Exercise 2: Finding CRISPR sites and checking for off-target matches
Exercise 3: Finding and filtering CRISPR sites and checking for off-target matches
Exercise 4: Finding paired CRISPR sites

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