Figure 14.9:
Batch golden gate cloning example with a list of codon-optimized CDS sequences
Batch Golden Gate cloning can be performed where one or more parts to be cloned is a list of sequences. Each of the individual nucleotide sequences in the list will be inserted into a separate product at the same insert position. If multiple sequence lists are used, all possible combinations of products will be output.
Note that the sequences within each list must be in the same orientation and must use the same overhangs.
Sequence lists are represented by the brown tags in the construct layout panel, and the number of products that will be generated is listed below the viewer window. Products can optionally be saved in a sequence list, by ticking the option save results as list.