Geneious contains a built-in list of common ladders which can be selected from the Ladders drop down menu to the right of the viewer. To add another ladder, click the ? next to the Ladders menu, then Edit Ladders File. This brings up a text editor where you can add your ladder to the existing ladders file.
Ladders must be added in the following format:
Name: BP or KBP: fragment sizes separated by commas
For example:
10bp DNA Ladder : BP : 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 250, 330
Note that fragment sizes must always be listed in base pairs. The designation BP or KBP refers only to the display (KBP looks better for large numbers over 100 kbp). Once you have added the ladder you want, click Save to record the changes.