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11.2
Analyzing Expression Levels
11.2.1
Calculating Expression Levels
Counting
RPKM
FPKM
TPM
Results
11.2.2
Comparing Expression Levels
11.2.3
Geneious Method for Comparing Expression Levels
Normalization
P-Value Calculation
11.2.4
DESeq2 Method for Comparing Expression Levels
Fit Type
Assigning Samples to Conditions
11.2.5
Results
11.2.6
Principal Component Analysis for DESeq2 results
Creating a PCA Plot
Interpreting PCA Plots
An Example of Using PCA As An Exploratory Tool
11.2.7
Volcano Plots
11.2.8
Comparing Expression Levels Using the DESeq2 Method on Linux Systems
Installing R
Installing DESeq2
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