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Chapter 16
BLAST

BLAST stands for Basic Local Alignment Search Tool (Altschul et al 1990). It allows you to query a sequence database with a sequence in order to find entries in the database that contain similar sequences. When “BLAST-ing”, you are able to specify either nucleotide or protein sequences and nucleotide sequences can be either DNA or RNA sequences. Sequences can be BLAST-ed against databases held at NCBI (see NCBI BLAST), or contained within your local Geneious database (Custom BLAST).

  16.1 Setting up a BLAST search
  16.2 BLAST results
   16.2.1 BLAST hit table
   16.2.2 Query-centric view
  16.3 NCBI BLAST
   16.3.1 Edit BLAST Databases
  16.4 Adding alternative BLAST server locations
  16.5 Custom BLAST
   16.5.1 Setting up the Custom BLAST files through Geneious Prime
   16.5.2 Setting up the Custom BLAST files yourself
   16.5.3 Adding Databases
   16.5.4 Using Custom BLAST

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