14.2.1 Direct extraction of restriction fragment

To extract the restriction digest product between two cut sites, click on the first site, then hold down the shift key and click on the second site. This will select the region between the sites. Then click the Extract button. You will be given the option to either extract the Digested fragment, or the Selected region. If Digested fragment is selected, the resulting product will contain the restriction site overhangs produced by the digestion.


Figure 14.3: Direct extraction of a restriction site product, showing (A) selection between two restriction sites, (B) the Extract dialog for extraction of the digested product, and (C) restriction site overhangs on the resulting product