14.9 TOPO® Cloning

TOPO Cloning lets you ligate a single fragment into a Vector within only 5 minutes using the natural activity of Topoisomerase I which recognizes a specific motif 5- (C/T)CCTT - 3on the DNA. TOPO is a registered trademark of Invitrogen Corporation.

The TOPO cloning function under the Cloning menu allows you to insert linear fragments into either linear TOPO vectors (when a TOPO-site is present at the extremities) or into circular TOPO vectors. You can select as many sequences at once as you like, they will be ligated into each other in a batch operation.


Figure 14.5: TOPO Cloning options dialog.